| First Author | Kumar KG | Year | 2008 |
| Journal | J Nutrigenet Nutrigenomics | Volume | 1 |
| Issue | 4 | Pages | 155-71 |
| PubMed ID | 19776624 | Mgi Jnum | J:153647 |
| Mgi Id | MGI:4365900 | Doi | 10.1159/000113657 |
| Citation | Kumar KG, et al. (2008) Transcriptional profiling of Chromosome 17 QTL for carbohydrate and total calorie intake in a mouse congenic strain reveals candidate genes and pathways. J Nutrigenet Nutrigenomics 1(4):155-171 |
| abstractText | BACKGROUND/AIMS: The genetic basis for ingestive behaviors is virtually unknown. Quantitative trait loci (QTLs) for carbohydrate and energy intake map to mouse chromosome 17 and were previously confirmed by a congenic strain bearing CAST/Ei (CAST) donor segment on the C57BL/6J (B6) background. METHODS: We used microarray technology to facilitate gene identification. Gene expression was compared between the B6.CAST-17 (BC-17) congenic and B6 strains in two diets: 1) chow, and 2) carbohydrate/protein vs. fat/protein. RESULTS: Within the QTL and unique to macronutrient selection, Agpat1 (acylglycerol-3-phosphate O-acyltransferase 1) was differentially expressed in hypothalamus. Irrespective of diet, the gene with the highest fold difference in congenic mice was trefoil factor 3 (Tff3) in liver. Several genes involved in fat metabolism were decreased in carbohydrate-preferring congenic mice, while genes associated with carbohydrate metabolism were increased. In particular, the glyoxalase pathway was enhanced including Glo1, Glo2, and dLDH. Higher expression of Glo1 mRNA in BC-17 congenic mice corresponded to increased protein expression revealed by Western blot, and to higher GLO1 activity in blood. CONCLUSION: These genes represent new candidates for nutrient intake phenotypes. We propose that increased GLO1 in the BC-17 strain supports its need to protect against dietary oxidants resulting from high carbohydrate intake. |
