| First Author | Yang J | Year | 2020 |
| Journal | Stem Cells | Volume | 38 |
| Issue | 4 | Pages | 556-573 |
| PubMed ID | 31721359 | Mgi Jnum | J:324856 |
| Mgi Id | MGI:6707506 | Doi | 10.1002/stem.3122 |
| Citation | Yang J, et al. (2020) A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3gamma phosphorylation. Stem Cells 38(4):556-573 |
| abstractText | Histone deacetylase 7 (HDAC7) plays a pivotal role in the maintenance of the endothelium integrity. In this study, we demonstrated that the intron-containing Hdac7 mRNA existed in the cytosol and that ribosomes bound to a short open reading frame (sORF) within the 5'-terminal noncoding area of this Hdac7 mRNA in response to vascular endothelial growth factor (VEGF) stimulation in the isolated stem cell antigen-1 positive (Sca1(+) ) vascular progenitor cells (VPCs). A 7-amino acid (7A) peptide has been demonstrated to be translated from the sORF in Sca1(+) -VPCs in vitro and in vivo. The 7A peptide was shown to receive phosphate group from the activated mitogen-activated protein kinase MEKK1 and transfer it to 14-3-3 gamma protein, forming an MEKK1-7A-14-3-3gamma signal pathway downstream VEGF. The exogenous synthetic 7A peptide could increase Sca1(+) -VPCs cell migration, re-endothelialization in the femoral artery injury, and angiogenesis in hind limb ischemia. A Hd7-7sFLAG transgenic mice line was generated as the loss-of-function model, in which the 7A peptide was replaced by a FLAG-tagged scrabbled peptide. Loss of the endogenous 7A impaired Sca1(+) -VPCs cell migration, re-endothelialization of the injured femoral artery, and angiogenesis in ischemic tissues, which could be partially rescued by the addition of the exogenous 7A/7Ap peptide. This study provides evidence that sORFs can be alternatively translated and the derived peptides may play an important role in physiological processes including vascular remodeling. |
