| First Author | Leong JW | Year | 2016 |
| Journal | Neural Plast | Volume | 2016 |
| Pages | 1951250 | PubMed ID | 27034842 |
| Mgi Jnum | J:306149 | Mgi Id | MGI:6709181 |
| Doi | 10.1155/2016/1951250 | Citation | Leong JW, et al. (2016) Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain. Neural Plast 2016:1951250 |
| abstractText | MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. In situ hybridization analysis showed that miR-344b and miR-344c were expressed in the germinal layer during embryonic brain developmental stages. In contrast, miR-344b was not detectable in the adult brain while miR-344c was expressed exclusively in the adult olfactory bulb and cerebellar granular layer. Stem-loop RT-qPCR analysis of whole brain RNAs showed that expression of the miR-344b and miR-344c was increased as brain developed throughout the embryonic stage and maintained at adulthood. Further investigation showed that these miRNAs were expressed in adult organs, where miR-344b and miR-344c were highly expressed in pancreas and brain, respectively. Bioinformatics analysis suggested miR-344b and miR-344c targeted Olig2 and Otx2 mRNAs, respectively. However, luciferase experiments demonstrated that these miRNAs did not target Olig2 and Otx2 mRNAs. Further investigation on the locality of miR-344b and miR-344c showed that both miRNAs were localized in nuclei of immature neurons. In conclusion, miR-344b and miR-344c were expressed spatiotemporally during mouse brain developmental stages. |
