| First Author | Li G | Year | 2021 |
| Journal | Nat Cell Biol | Volume | 23 |
| Issue | 5 | Pages | 526-537 |
| PubMed ID | 33958760 | Mgi Jnum | J:343518 |
| Mgi Id | MGI:6709606 | Doi | 10.1038/s41556-021-00672-3 |
| Citation | Li G, et al. (2021) LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy. Nat Cell Biol |
| abstractText | Major histocompatibility complex-I (MHC-I) presents tumour antigens to CD8(+) T cells and triggers anti-tumour immunity. Humans may have 30,000-60,000 long noncoding RNAs (lncRNAs). However, it remains poorly understood whether lncRNAs affect tumour immunity. Here, we identify a lncRNA, lncRNA inducing MHC-I and immunogenicity of tumour (LIMIT), in humans and mice. We found that IFNgamma stimulated LIMIT, LIMIT cis-activated the guanylate-binding protein (GBP) gene cluster and GBPs disrupted the association between HSP90 and heat shock factor-1 (HSF1), thereby resulting in HSF1 activation and transcription of MHC-I machinery, but not PD-L1. RNA-guided CRISPR activation of LIMIT boosted GBPs and MHC-I, and potentiated tumour immunogenicity and checkpoint therapy. Silencing LIMIT, GBPs and/or HSF1 diminished MHC-I, impaired antitumour immunity and blunted immunotherapy efficacy. Clinically, LIMIT, GBP- and HSF1-signalling transcripts and proteins correlated with MHC-I, tumour-infiltrating T cells and checkpoint blockade response in patients with cancer. Together, we demonstrate that LIMIT is a cancer immunogenic lncRNA and the LIMIT-GBP-HSF1 axis may be targetable for cancer immunotherapy. |
