| First Author | Crozet F | Year | 2021 |
| Journal | Development | Volume | 148 |
| Issue | 7 | PubMed ID | 33722900 |
| Mgi Jnum | J:305438 | Mgi Id | MGI:6710312 |
| Doi | 10.1242/dev.199364 | Citation | Crozet F, et al. (2021) Myosin-X is dispensable for spindle morphogenesis and positioning in the mouse oocyte. Development 148(7):dev199364 |
| abstractText | Off-center spindle positioning in mammalian oocytes enables asymmetric divisions in size, which are important for subsequent embryogenesis. The migration of the meiosis I spindle from the oocyte center to its cortex is mediated by F-actin. Specifically, an F-actin cage surrounds the microtubule spindle and applies forces to it. To better understand how F-actin transmits forces to the spindle, we studied a potential direct link between F-actin and microtubules. For this, we tested the implication of myosin-X, a known F-actin and microtubule binder involved in spindle morphogenesis and/or positioning in somatic cells, amphibian oocytes and embryos. Using a mouse strain conditionally invalidated for myosin-X in oocytes and by live-cell imaging, we show that myosin-X is not localized on the spindle, and is dispensable for spindle and F-actin assembly. It is not required for force transmission as spindle migration and chromosome alignment occur normally. More broadly, myosin-X is dispensable for oocyte developmental potential and female fertility. We therefore exclude a role for myosin-X in transmitting F-actin-mediated forces to the spindle, opening new perspectives regarding this mechanism in mouse oocytes, which differ from most mitotic cells. |
