| First Author | Xiang L | Year | 2020 |
| Journal | Stem Cell Res Ther | Volume | 11 |
| Issue | 1 | Pages | 403 |
| PubMed ID | 32943107 | Mgi Jnum | J:309399 |
| Mgi Id | MGI:6757608 | Doi | 10.1186/s13287-020-01928-9 |
| Citation | Xiang L, et al. (2020) FOXQ1 promotes the osteogenic differentiation of bone mesenchymal stem cells via Wnt/beta-catenin signaling by binding with ANXA2. Stem Cell Res Ther 11(1):403 |
| abstractText | BACKGROUND: This study investigated the role of Forkhead box Q1 (FOXQ1) in the osteogenic differentiation of bone mesenchymal stem cells. METHODS: Mouse bone mesenchymal stem cells (mBMSCs) were transfected with lentivirus to generate Foxq1-overexpressing mBMSCs, Foxq1-suppressed mBMSCs, and mBMSC controls. The activity of osteogenic differentiation was evaluated with alizarin red staining, alkaline phosphatase activity assay, and RT-qPCR. Wnt/beta-catenin signaling activities were compared among groups by TOPFlash/FOPFlash assay, immunofluorescence staining, and western blot assay of beta-catenin (CTNNB1). Coimmunoprecipitation mass spectrometry was also carried out to identify proteins binding with FOXQ1. RESULTS: Our data showed that FOXQ1 expression was positively correlated with the osteogenic differentiation of the mBMSCs. FOXQ1 also promoted the nuclear translocation of CTNNB1 in the mBMSCs, enhancing Wnt/beta-catenin signaling, which was also shown to be essential for the osteogenic differentiation-promoting effect of FOXQ1 in the mBMSCs. Annexin A2 (ANXA2) was bound with FOXQ1, and its depletion reversed the promoting effect of FOXQ1 on Wnt/beta-catenin signaling. CONCLUSION: These results showed that FOXQ1 binds with ANXA2, promoting Wnt/beta-catenin signaling in bone mesenchymal stem cells, which subsequently promotes osteogenic differentiation. |
