| First Author | Richter M | Year | 2014 |
| Journal | J Toxicol Sci | Volume | 39 |
| Issue | 2 | Pages | 353-61 |
| PubMed ID | 24646717 | Mgi Jnum | J:312152 |
| Mgi Id | MGI:6783422 | Doi | 10.2131/jts.39.353 |
| Citation | Richter M, et al. (2014) Pau d'arco activates Nrf2-dependent gene expression via the MEK/ERK-pathway. J Toxicol Sci 39(2):353-361 |
| abstractText | Pau dâarco is a plant-derived traditional medicine that acts by poorly understood molec-ular mechanisms. Here, we studied the effect of pau dâarco on the cytoprotective transcription factor Nrf2. An aqueous extract of pau dâarco stimulated Nrf2-dependent gene expression and led to nuclear localiza-tion of Nrf2 in vitro. Chromatographic separation and mass spectrometry of the extract identiï¬ed ben-zene trioles or benzene tetraoles within the active fractions. The extract stimulated the mitogen-activat-ed protein kinase/extracellular-signal-regulated kinase kinase (MEK)/extracellular-signal-regulated kinase (ERK1/2) pathway. The pharmacological inhibition of MEK, but not of p38 mitogen-activated protein kinase, glycogen synthase kinase-3 or phosphoinositide 3-kinase was required for the activation of Nrf2-dependent gene expression by pau dâarco, but not for the nuclear translocation of Nrf2. In vivo pau dâarco increased the expression of Nrf2-target genes in the intestine. The results suggest that the activation of Nrf2 could mediate beneï¬cial effects of pau dâarco, in particular in the intestine. |
