| First Author | Liu Y | Year | 2021 |
| Journal | J Mol Histol | Volume | 52 |
| Issue | 5 | Pages | 965-973 |
| PubMed ID | 34405343 | Mgi Jnum | J:314924 |
| Mgi Id | MGI:6828670 | Doi | 10.1007/s10735-021-10014-0 |
| Citation | Liu Y, et al. (2021) Nuclear-localized eukaryotic translation initiation factor 1A is involved in mouse preimplantation embryo development. J Mol Histol 52(5):965-973 |
| abstractText | Preimplantation embryo development is characterized by drastic nuclear reprogramming and dynamic stage-specific gene expression. Key regulators of this earliest developmental stage have not been revealed. In the present study, a "non-classical" nuclear-localization pattern of eIF1A was observed during early developmental stages of mouse preimplantation embryo before late-morula. In particular, eIF1A is most highly expressed in the nuclear of 2-cell embryo. Knockdown eIF1A by siRNA microinjection affected the development of mouse preimplantation embryo, resulted in decreased blastocyst formation rate. CDX2 protein expression level significantly down-regulated after eIF1A knockdown in morula stage. In addition, the mRNA expression level of Hsp70.1 was also decreased in 2-cell embryo. The results indicate an indispensable role of eIF1A in mouse preimplantation embryos. |
