| First Author | Giam M | Year | 2012 |
| Journal | Cell Death Dis | Volume | 3 |
| Pages | e378 | PubMed ID | 22914326 |
| Mgi Jnum | J:315245 | Mgi Id | MGI:6829892 |
| Doi | 10.1038/cddis.2012.117 | Citation | Giam M, et al. (2012) Detection of Bcl-2 family member Bcl-G in mouse tissues using new monoclonal antibodies. Cell Death Dis 3:e378 |
| abstractText | Bcl-G is an evolutionarily conserved member of the Bcl-2 family of proteins that has been implicated in regulating apoptosis and cancer. We have generated monoclonal antibodies that specifically recognise mouse Bcl-G and have used these reagents to analyse its tissue distribution and subcellular localisation using western blotting, immunohistochemistry and immunofluorescence. We found that Bcl-G predominantly resides in the cytoplasm and is present in a wide range of mouse tissues, including the spleen, thymus, lung, intestine and testis. Immunohistochemical analyses revealed that Bcl-G is expressed highly in mature spermatids in the testis, CD8(+) conventional dendritic cells (DCs) in hematopoietic tissues and diverse epithelial cell types, including those lining the gastrointestinal and respiratory tracts. The Bcl-G monoclonal antibodies represent new tools for studying this protein, using a variety of techniques, including immunoprecipitation and flow cytometry. |
