| First Author | Xu X | Year | 2017 |
| Journal | Neuroscience | Volume | 340 |
| Pages | 232-242 | PubMed ID | 27984181 |
| Mgi Jnum | J:310133 | Mgi Id | MGI:6759087 |
| Doi | 10.1016/j.neuroscience.2016.10.055 | Citation | Xu X, et al. (2017) AP-2alpha and AP-2beta regulate dorsal interneuron specification in the spinal cord. Neuroscience 340:232-242 |
| abstractText | To date, five AP-2 genes that encode AP-2alpha, beta, gamma, delta and epsilon have been identified in vertebrates and they have been reported to be key regulators of embryonic development. However, the role of AP-2 family members in the development of central nervous system (CNS) has not been characterized. In the present study, we systematically examined the spatiotemporal expression pattern of AP-2 genes in the developing spinal cord of mouse and chick embryos and found that AP-2alpha and AP-2beta are specifically expressed in post-mitotic dorsal interneurons. Loss-of-function analysis using in ovo electroporation in embryonic chick spinal cord preliminarily demonstrated that cAP-2alpha and cAP-2beta regulates dorsal Class A and Class B interneuron specification, respectively. Gain-of-function experiments further revealed that misexpression of cAP-2alpha, but not cAP-2beta, was able to induce the ectopic generation of Class A interneurons. Together, our studies indicated that AP-2 family members, AP-2alpha and AP-2beta, have distinct functions in the regulation of dorsal interneuron development. |
