| First Author | Kim JH | Year | 2014 |
| Journal | Cell Immunol | Volume | 289 |
| Issue | 1-2 | Pages | 55-62 |
| PubMed ID | 24727157 | Mgi Jnum | J:319033 |
| Mgi Id | MGI:6862420 | Doi | 10.1016/j.cellimm.2014.03.008 |
| Citation | Kim JH, et al. (2014) Transglutaminase 2 on the surface of dendritic cells is proposed to be involved in dendritic cell-T cell interaction. Cell Immunol 289(1-2):55-62 |
| abstractText | Transglutaminase 2 (TG2) is a ubiquitous enzyme involved in diverse biological processes. Recently, its function in adaptive immune responses has begun to emerge. Its presence and functions in B cells and T cells, for example, have been reported. However, those in dendritic cells (DCs), the principal antigen-presenting cells, are as yet unexplored in murine system. In this study, we first investigated the expression of TG2 in murine bone marrow-derived DCs, and then compared the functioning of these cells in the presence or absence of this enzyme using wild-type (WT) and TG2(-/-) mice. We found that the WT DCs expressed TG2 both in the cytoplasm and on the cell surface, both of which were elevated after LPS stimulation. Unexpectedly, between WT and TG2(-/-) DCs, there were no remarkable differences in cytokine secretion, IL-10 and IL-12, and neither in the expression of surface molecules CD80, CD86, and MHC II, excepting a moderate decrease of CD40 expression on the TG2(-/-) DCs. However, when T cells were stimulated with TG2(-/-) DCs, they showed decreased levels of proliferation, CD69 and CD25 expression, and IFN-gamma secretion. The addition of anti-TG2 antibody to the WT DC-T cell co-culture resulted in decreased T cell activation. By immunofluorescence staining, TG2 was observed at DC-T cell interface (contact point). Taken together, we propose that TG2 on the surface of DCs modulates the DC-T cell interaction. |
