| First Author | Kim MN | Year | 2014 |
| Journal | World J Gastroenterol | Volume | 20 |
| Issue | 45 | Pages | 17084-91 |
| PubMed ID | 25493020 | Mgi Jnum | J:319140 |
| Mgi Id | MGI:6862854 | Doi | 10.3748/wjg.v20.i45.17084 |
| Citation | Kim MN, et al. (2014) Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice. World J Gastroenterol 20(45):17084-91 |
| abstractText | AIM: To investigate the effect of Clostridium difficile (C. difficile) infection in an interleukin 10-deficient (IL-10(-/-)) mouse model of inflammatory bowel disease. METHODS: Bone marrow-derived dendritic cells isolated from wild type (WT) and IL-10(-/-)mice were stimulated for 4 h with C. difficile toxin A (200 mug/mL), and gene expression of interferon (IFN)-gamma, IL-12 and IL-23 was determined by real-time reverse transcription polymerase chain reaction. WT and IL-10(-/-) mice (n = 20 each) were exposed to an antibiotic cocktail for three days and then were injected with clindamycin (i.p.). Mice (n = 10 WT, 10 IL-10(-/-)) were then challenged with oral administration of C. difficile (1 x 10(5) colony forming units of strain VPI 10463). Animals were monitored daily for 7 d for signs of colitis. Colonic tissue samples were evaluated for cytokine gene expression and histopathologic analysis. RESULTS: C. difficile toxin A treatment induced IFN-gamma gene expression to a level that was significantly higher in BDMCs from IL-10(-/-) compared to those from WT mice (P < 0.05). However, expression of IL-12 and IL-23 was not different among the groups. Following C. difficile administration, mice developed diarrhea and lost weight within 2-3 d. Weight loss was significantly greater in IL-10(-/-) compared to WT mice (P < 0.05). C. difficile infection induced histopathologic features typical of colitis in both IL-10(-/-) and WT mice. The histopathologic severity score was significantly higher in the IL-10(-/-) than in WT mice (mean +/- standard error; 5.50 +/- 0.53 vs 2.44 +/- 0.46; P < 0.05). This was accompanied by a significantly greater increase in IFN-gamma gene expression in colonic tissues from IL-10(-/-) than from WT mice challenged with C. difficile (P < 0.05). CONCLUSION: These results indicate that colitis is more severe after C. difficile infection in IL-10(-/-)mice, and that IFN-gamma expression is involved in this process. |
