| First Author | Hiramatsu-Asano S | Year | 2020 |
| Journal | Front Immunol | Volume | 11 |
| Pages | 616141 | PubMed ID | 33574820 |
| Mgi Jnum | J:329684 | Mgi Id | MGI:6804766 |
| Doi | 10.3389/fimmu.2020.616141 | Citation | Hiramatsu-Asano S, et al. (2020) Deletion of Mir223 Exacerbates Lupus Nephritis by Targeting S1pr1 in Fas(lpr/lpr) Mice. Front Immunol 11:616141 |
| abstractText | Objective: The micro RNAs (miRNAs) and their target mRNAs are differentially expressed in various immune-mediated cells. Here, we investigated the role of Mir223 and sphingosine-1-phosphate receptor 1 (S1pr1) in the pathogenesis of systemic lupus erythematosus. Methods: We analyzed miRNA and mRNA profiling data of CD4(+) splenic T cells derived from MRL/MpJ-Fas(lpr) /J mice. We performed 3' untranslated region (UTR) luciferase reporter gene assay using human umbilical vein endothelial cells (HUVECs). We generated the B6-Mir223 (-/-) Fas(lpr/lpr) mice and the lupus phenotypes were analyzed. Results: In CD4(+) splenic T cells, we identified upregulation of miR-223-3p and downregulation of the possible target, S1pr1 by RNA sequencing of MRL/MpJ-Fas(lpr) /J mice. The transfection with miR-223-3p mimic significantly suppressed a luciferase activity in HUVEC treated with a Lentivirus vector containing 3' UTR of S1pr1. The mRNA levels of S1pr1 were significantly decreased after miR-223-3p overexpression. In B6-Mir223 (-/-) Fas(lpr/lpr) mice, the proportion of CD3(+) T cells, CD3(+)CD4(-)CD8(-) cells, B cells, plasma cells, and S1PR1(+)CD4(+) T cells in the spleen was significantly increased compared with that in B6-Mir223 (+/+) Fas(lpr/lpr) mice by flow cytometry. B6-Mir223 (-/-) Fas(lpr/lpr) mice demonstrated the elevation of glomerular and renal vascular scores associated with enhanced intraglomerular infiltration of S1PR1(+)CD4(+) T cells. Conclusion: Unexpectedly, the deletion of Mir223 exacerbated the lupus phenotypes associated with increased population of S1PR1(+)CD4(+) T in spleen and the enhanced infiltration of S1PR1(+)CD4(+) T cells in inflamed kidney tissues, suggesting compensatory role of Mir223 in the pathogenesis of lupus nephritis. |
