| First Author | Sun YF | Year | 2018 |
| Journal | Mol Med Rep | Volume | 17 |
| Issue | 4 | Pages | 5988-5995 |
| PubMed ID | 29436664 | Mgi Jnum | J:317612 |
| Mgi Id | MGI:6856332 | Doi | 10.3892/mmr.2018.8602 |
| Citation | Sun YF, et al. (2018) Knockout of microRNA26a promotes lung development and pulmonary surfactant synthesis. Mol Med Rep 17(4):5988-5995 |
| abstractText | Normal formation and function of the lungs are essential for the transition of the fetus to an airbreathing environment at birth. The synthesis of pulmonary surfactant (PS), which is produced by type II alveolar epithelial cells (AECIIs), is required for proper lung development. Previous in vitro studies have suggested that PS synthesis is regulated by microRNA (miR)26a in fetal rat AECIIs. The present study explored the potential role of miR26a in lung development and PS synthesis by using a miR26a1/miR26a2 double knockout mouse model. Hematoxylin and eosin staining and transmission electron microscopy were used to observe the morphology of fetal lungs. Reverse transcriptionquantitative polymerase chain reaction and western blot analysis were performed to examine the mRNA and protein levels of surfactantassociated proteins. The results demonstrated that the lung formation in the knockout mice was more mature, and that there were more mature lamellar bodies inside AECIIs in miR26a knockout mice at late stages of lung development. The findings further demonstrated that knockout of miR26a increased surfactantassociated mRNA and protein expression levels. The results indicated that knockout of miR26a promotes lung development and PS synthesis. |
