| First Author | Burke SJ | Year | 2016 |
| Journal | Biofactors | Volume | 42 |
| Issue | 6 | Pages | 703-715 |
| PubMed ID | 27325565 | Mgi Jnum | J:318385 |
| Mgi Id | MGI:6859454 | Doi | 10.1002/biof.1304 |
| Citation | Burke SJ, et al. (2016) Pancreatic beta-Cell production of CXCR3 ligands precedes diabetes onset. Biofactors 42(6):703-715 |
| abstractText | Type 1 diabetes mellitus (T1DM) results from immune cell-mediated reductions in function and mass of the insulin-producing beta-cells within the pancreatic islets. While the initial trigger(s) that initiates the autoimmune process is unknown, there is a leukocytic infiltration that precedes islet beta-cell death and dysfunction. Herein, we demonstrate that genes encoding the chemokines CXCL9, 10, and 11 are primary response genes in pancreatic beta-cells and are also elevated as part of the inflammatory response in mouse, rat, and human islets. We further established that STAT1 participates in the transcriptional control of these genes in response to the pro-inflammatory cytokines IL-1beta and IFN-gamma. STAT1 is phosphorylated within five minutes after beta-cell exposure to IFN-gamma, with subsequent occupancy at proximal and distal response elements within the Cxcl9 and Cxcl11 gene promoters. This increase in STAT1 binding is coupled to the rapid appearance of chemokine transcript. Moreover, circulating levels of chemokines that activate CXCR3 are elevated in non-obese diabetic (NOD) mice, consistent with clinical findings in human diabetes. We also report herein that mice with genetic deletion of CXCR3 (receptor for ligands CXCL9, 10, and 11) exhibit a delay in diabetes development after being injected with multiple low doses of streptozotocin. Therefore, we conclude that production of CXCL9, 10, and 11 from islet beta-cells controls leukocyte migration and activity into pancreatic tissue, which ultimately influences islet beta-cell mass and function. (c) 2016 BioFactors, 42(6):703-715, 2016. |
