| First Author | Ito K | Year | 2018 |
| Journal | Mol Biol Cell | Volume | 29 |
| Issue | 2 | Pages | 209-219 |
| PubMed ID | 29142070 | Mgi Jnum | J:318513 |
| Mgi Id | MGI:6859941 | Doi | 10.1091/mbc.E17-05-0271 |
| Citation | Ito K, et al. (2018) Gfap and Osmr regulation by BRG1 and STAT3 via interchromosomal gene clustering in astrocytes. Mol Biol Cell 29(2):209-219 |
| abstractText | Long-range chromatin interactions between gene loci in the cell nucleus are important for many biological processes, including transcriptional regulation. Previously, we demonstrated that several genes specifically cluster with the astrocyte-specific gene for glial fibrillary acidic protein (Gfap) during astrocyte differentiation; however, the molecular mechanisms for gene clustering remain largely unknown. Here we show that brahma-related gene 1 (BRG1), an ATP-dependent chromatin remodeling factor, and the transcription factor STAT3 are required for Gfap and oncostatin M receptor (Osmr) clustering and enhanced expression through recruitment to STAT3 recognition sequences and that gene clustering occurs prior to transcriptional up-regulation. BRG1 knockdown and JAK-STAT signaling inhibition impaired clustering, leading to transcriptional down-regulation of both genes. BRG1 and STAT3 were recruited to the same Gfap fragment; JAK-STAT signaling inhibition impaired BRG1 recruitment. Our results suggest that BRG1 and STAT3 coordinately regulate gene clustering and up-regulate Gfap and Osmr transcription. |
