| First Author | Negretti NM | Year | 2021 |
| Journal | Development | Volume | 148 |
| Issue | 24 | PubMed ID | 34927678 |
| Mgi Jnum | J:316879 | Mgi Id | MGI:6842889 |
| Doi | 10.1242/dev.199512 | Citation | Negretti NM, et al. (2021) A single-cell atlas of mouse lung development. Development 148(24):dev199512 |
| abstractText | Lung organogenesis requires precise timing and coordination to effect spatial organization and function of the parenchymal cells. To provide a systematic broad-based view of the mechanisms governing the dynamic alterations in parenchymal cells over crucial periods of development, we performed a single-cell RNA-sequencing time-series yielding 102,571 epithelial, endothelial and mesenchymal cells across nine time points from embryonic day 12 to postnatal day 14 in mice. Combining computational fate-likelihood prediction with RNA in situ hybridization and immunofluorescence, we explore lineage relationships during the saccular to alveolar stage transition. The utility of this publicly searchable atlas resource (www.sucrelab.org/lungcells) is exemplified by discoveries of the complexity of type 1 pneumocyte function and characterization of mesenchymal Wnt expression patterns during the saccular and alveolar stages - wherein major expansion of the gas-exchange surface occurs. We provide an integrated view of cellular dynamics in epithelial, endothelial and mesenchymal cell populations during lung organogenesis. |
