| First Author | Zhou Y | Year | 2017 |
| Journal | J Cell Physiol | Volume | 232 |
| Issue | 11 | Pages | 3050-3059 |
| PubMed ID | 27509531 | Mgi Jnum | J:328376 |
| Mgi Id | MGI:6871301 | Doi | 10.1002/jcp.25516 |
| Citation | Zhou Y, et al. (2017) DJ-1/Park7 Sensitive Na(+) /H(+) Exchanger 1 (NHE1) in CD4(+) T Cells. J Cell Physiol 232(11):3050-3059 |
| abstractText | DJ-1/Park7 is a redox-sensitive chaperone protein counteracting oxidation and presumably contributing to the control of oxidative stress responses and thus inflammation. DJ-1 gene deletion exacerbates the progression of Parkinson's disease presumably by augmenting oxidative stress. Formation of reactive oxygen species (ROS) is paralleled by activation of the Na(+) /H(+) exchanger 1 (NHE1). ROS formation in CD4(+) T cells plays a decisive role in regulating inflammatory responses. In the present study, we explored whether DJ-1 is expressed in CD4(+) T cells, and affects ROS production as well as NHE1 in those cells. To this end, DJ-1 and NHE1 transcript, and protein levels were quantified by qRT-PCR and Western blotting, respectively, intracellular pH (pHi ) utilizing bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence, NHE activity from realkalinization after an ammonium pulse, and ROS production utilizing 2',7' -dichlorofluorescin diacetate (DCFDA) fluorescence. As a result DJ-1 was expressed in CD4(+) T cells. ROS formation, NHE1 transcript levels, NHE1 protein, and NHE activity were higher in CD4(+) T cells from DJ-1 deficient mice than in CD4(+) T cells from wild type mice. Antioxidant N-acetyl-cysteine (NAC) and protein tyrosine kinase (PTK) inhibitor staurosporine decreased the NHE activity in DJ-1 deficient CD4(+) T cells, and blunted the difference between DJ-1(-/-) and DJ-1(+/+) CD4(+) T cells, an observation pointing to a role of ROS in the up-regulation of NHE1 in DJ-1(-/-) CD4(+) T cells. In conclusion, DJ-1 is a powerful regulator of ROS production as well as NHE1 expression and activity in CD4(+) T cells. J. Cell. Physiol. 232: 3050-3059, 2017. (c) 2016 Wiley Periodicals, Inc. |
