| First Author | Newlaczyl AU | Year | 2017 |
| Journal | Sci Rep | Volume | 7 |
| Pages | 41297 | PubMed ID | 28117393 |
| Mgi Jnum | J:320537 | Mgi Id | MGI:6873655 |
| Doi | 10.1038/srep41297 | Citation | Newlaczyl AU, et al. (2017) Quantification of spatiotemporal patterns of Ras isoform expression during development. Sci Rep 7:41297 |
| abstractText | Ras proteins are important signalling hubs frequently dysregulated in cancer and in a group of developmental disorders called Rasopathies. Three Ras genes encode four proteins that differentially contribute to these phenotypes. Using quantitative real-time PCR (qRT-PCR) we have measured the gene expression profiles of each of the Ras isoforms in a panel of mouse tissues derived from a full developmental time course spanning embryogenesis through to adulthood. In most tissues and developmental stages we observe a relative contribution of KRas4B > > NRas >/= KRas4A > HRas to total Ras expression with KRas4B typically representing 60-99% of all Ras transcripts. KRas4A is the most dynamically regulated Ras isoform with significant up-regulation of expression observed pre-term in stomach, intestine, kidney and heart. The expression patterns assist interpretation of the essential role of KRas in development and the preponderance of KRas mutations in cancer. |
