| First Author | Kamal A | Year | 2000 |
| Journal | Neuron | Volume | 28 |
| Issue | 2 | Pages | 449-59 |
| PubMed ID | 11144355 | Mgi Jnum | J:381507 |
| Mgi Id | MGI:8297735 | Doi | 10.1016/s0896-6273(00)00124-0 |
| Citation | Kamal A, et al. (2000) Axonal transport of amyloid precursor protein is mediated by direct binding to the kinesin light chain subunit of kinesin-I. Neuron 28(2):449-59 |
| abstractText | We analyzed the mechanism of axonal transport of the amyloid precursor protein (APP), which plays a major role in the development of Alzheimer's disease. Coimmunoprecipitation, sucrose gradient, and direct in vitro binding demonstrated that APP forms a complex with the microtubule motor, conventional kinesin (kinesin-I), by binding directly to the TPR domain of the kinesin light chain (KLC) subunit. The estimated apparent Kd for binding is 15-20 nM, with a binding stoichiometry of two APP per KLC. In addition, association of APP with microtubules and axonal transport of APP is greatly decreased in a gene-targeted mouse mutant of the neuronally enriched KLC1 gene. We propose that one of the normal functions of APP may be as a membrane cargo receptor for kinesin-I and that KLC is important for kinesin-I-driven transport of APP into axons. |
