|  Help  |  About  |  Contact Us

Publication : Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays.

First Author  Chen A Year  2022
Journal  Cell Volume  185
Issue  10 Pages  1777-1792.e21
PubMed ID  35512705 Mgi Jnum  J:325251
Mgi Id  MGI:7281611 Doi  10.1016/j.cell.2022.04.003
Citation  Chen A, et al. (2022) Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays. Cell 185(10):1777-1792.e21
abstractText  Spatially resolved transcriptomic technologies are promising tools to study complex biological processes such as mammalian embryogenesis. However, the imbalance between resolution, gene capture, and field of view of current methodologies precludes their systematic application to analyze relatively large and three-dimensional mid- and late-gestation embryos. Here, we combined DNA nanoball (DNB)-patterned arrays and in situ RNA capture to create spatial enhanced resolution omics-sequencing (Stereo-seq). We applied Stereo-seq to generate the mouse organogenesis spatiotemporal transcriptomic atlas (MOSTA), which maps with single-cell resolution and high sensitivity the kinetics and directionality of transcriptional variation during mouse organogenesis. We used this information to gain insight into the molecular basis of spatial cell heterogeneity and cell fate specification in developing tissues such as the dorsal midbrain. Our panoramic atlas will facilitate in-depth investigation of longstanding questions concerning normal and abnormal mammalian development.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

48 Bio Entities

Trail: Publication

0 Expression