| First Author | Tian Y | Year | 2022 |
| Journal | Front Genet | Volume | 13 |
| Pages | 909095 | PubMed ID | 35801079 |
| Mgi Jnum | J:332327 | Mgi Id | MGI:7310671 |
| Doi | 10.3389/fgene.2022.909095 | Citation | Tian Y, et al. (2022) LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1alpha. Front Genet 13:909095 |
| abstractText | Background: Osteoclasts, which are multinucleated cells formed by monocyte fusion, play a key role in bone resorption. Hypoxia-inducible factor (HIF)-1alpha is vital for the development of osteoclasts in hypoxic environments and during bone resorption. However, additional research is required to further study the HIF-1alpha-dependent regulation of osteoclast differentiation at the genetic level. Methods: In our study, RNA sequencing (RNA-seq) was used to identify the expression profiles of long noncoding RNAs (lncRNAs) and mRNAs in conditional HIF-1alpha-knockout osteoclasts. Results: A total of 1,320 mRNAs and 95 lncRNAs were differentially expressed. The expression of lncRNAs MSTRG.7566.12 and MSTRG.31769.2 were strongly negatively correlated with that of Mmp9, Ctsk, etc. Conclusion: Our research provides a basis for further understanding the role of mRNAs and lncRNAs in conditional HIF-1alpha-knockout osteoclasts, and many of these molecules may be potential targets for treating bone diseases related to HIF-1alpha. |
