| First Author | Scibiorek M | Year | 2023 |
| Journal | Sci Rep | Volume | 13 |
| Issue | 1 | Pages | 144 |
| PubMed ID | 36599893 | Mgi Jnum | J:351828 |
| Mgi Id | MGI:7424312 | Doi | 10.1038/s41598-022-26637-6 |
| Citation | Scibiorek M, et al. (2023) IL-4Ralpha signalling in B cells and T cells play differential roles in acute and chronic atopic dermatitis. Sci Rep 13(1):144 |
| abstractText | Atopic dermatitis (AD) is a common pruritic inflammatory skin disease with complex environmental and genetic predisposing factors. Primary skin barrier dysfunction and aberrant T helper 2 (TH2) responses to common allergens, together with increased serum IgE antibodies, characterise the disease. B and T cells are essential in the disease manifestation, however, the exact mechanism of how these cells is involved is unclear. Targeting interleukin 4 receptor alpha (IL-4Ralpha), an IL-4/IL-13 signalling axis, with dupilumab shows efficacy in AD. We investigated the importance of IL-4Ralpha signalling specifically on B and T cells during acute and chronic models of AD. We used House dust mite (HDM) and Ovalbumin (OVA) in chronic models and a low-calcemic analog of vitamin D (MC903) for acute models of AD. We used mb1(cre)IL-4Ralpha(-/lox), iLCK(cre)IL-4Ralpha(-/lox), LCK(cre)IL-4Ralpha(-/lox), CD4(cre)IL-4Ralpha(-/lox), Foxp3(cre)IL-4Ralpha(-/lox) and IL-4Ralpha(-/lox) littermate controls. IL-4Ralpha-responsive B cells were essential in serum IgE levels, but not in epidermal thickening in both chronic and acute models. IL-4Ralpha-responsive T cells were essential in epidermal thickening in the pan-T cell, but not CD4 or CD8 T cells suggesting the importance of gammadeltaT cells during acute AD. Our results suggest that IL-4Ralpha responsiveness on innate T cells regulates acute atopic dermatitis, while on B cells it regulates IgE. |
