| First Author | Sun T | Year | 2019 |
| Journal | Immunol Cell Biol | Volume | 97 |
| Issue | 6 | Pages | 552-562 |
| PubMed ID | 30768806 | Mgi Jnum | J:344052 |
| Mgi Id | MGI:7444900 | Doi | 10.1111/imcb.12244 |
| Citation | Sun T, et al. (2019) NLRC5 deficiency has a moderate impact on immunodominant CD8(+) T-cell responses during rotavirus infection of adult mice. Immunol Cell Biol 97(6):552-562 |
| abstractText | The NOD-like receptor (NLR) family plays an important role in innate immunity. Class II transactivator and NOD-like receptor caspase activation and recruitment domain CARD containing 5 (NLRC5) are unusual members of the NLR family that instead of recognizing pathogen-associated or damage-associated molecular patterns, form enhanceosomes with adaptor molecules and modulate major histocompatibility complex (MHC) class II and MHC class I expression, respectively. While NLRC5 has been shown to play a role during intracellular pathogen infection and tumor cell immune evasion, its role in regulating antigen-specific CD8(+) T-cell responses at the intestinal mucosa has not been investigated. Here, we take advantage of the rotavirus model in adult mice to dissect the impact of NLRC5 on CD8(+) T-cell responses to this viral infection at the gut mucosa. We show that while Nlrc5(-/-) mice exhibited normal proportions of T-cell subpopulations in the intraepithelial and lamina propria compartments, these mice had decreased baseline MHC class I expression on various immune cells in the lamina propria. Upon rotavirus infection, Nlrc5 deficiency resulted in impaired H2-K(b) -restricted antigen-specific CD8(+) T-cell responses, which were recapitulated in mice deficient for Nlrc5 within the dendritic cell compartment. The impaired CD8(+) T-cell response in Nlrc5(-/-) mice was not significant enough to impact viral titers, suggesting compensation in Nlrc5(-/-) mice, perhaps as a result of higher numbers of activated B cells in the mesenteric lymph nodes and normal rotavirus-specific immunoglobulin A responses. Collectively, our results demonstrate a minor role for NLRC5 in modulating H2-K(b) -restricted antigen-specific CD8(+) T-cell responses in the small intestine during rotavirus infection in adult mice. |
