| First Author | Scott D | Year | 1997 |
| Journal | Genet Res | Volume | 70 |
| Issue | 1 | Pages | 80-1 (Abstr.) |
| Mgi Jnum | J:46861 | Mgi Id | MGI:1327854 |
| Doi | 10.1017/S0016672397262865 | Citation | Scott D, et al. (1997) Identification of H-Y epitopes in Smcy and other genes mapping to the mouse Y Chromosome short arm. Genet Res 70(1):80-1 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of papers presented at the seventh Mammalian Genetics and Development Workshop held at the Wellcome Trust Building, Euston Road, London on 20-22 November 1996. Identification of H-Y epitopes in Smcy and other genes mapping to the mouse Y chromosome short arm D. SCOTT,l I. EHRMANN,1 P. ELLIS1, M. MITCHELL2, A. GREENFIELD3,4, P. KOOPMAN3 1Clinical Sciences Centre, RPMS, London, UK. 2Inserm Unite 406, Marseille, France. 3Centre for Molecular and Cellular Biology, Brisbane, Australia. 4MRC Mammalian Genetics Unit, Harwell, UK. Two H-Y epitopes - mouse and human HYB7 - were recently identified as products of Smcy, a gene mapping to the short arm of the mouse Y chromosome. The H-YKk epitope was defined as the peptide, TENSGKDI. This differs by five amino acids from the X chromosome homologue, which does not stimulate an H-Y specific T cell clone. Deletion mutants not expressing this peptide fail to stimulate the T clone. A 5 kb cDNA expressing most of the Smcy sequence was synthesized by PCR from a 5' Smcy cDNA clone. Following transfection, this gave rise to H- Ykk expression, as did a 3' cDNA clone which also encoded the peptide. Smcy was also found to express an H-YDk epitope, which has been localized to two exons mapping within a 9 kb genomic fragment (MEM 14 B) that lies 5' of that expressing the H-YKk. Smcy does not appear to encode other H-Y epitopes, suggesting that there are other genes in this region from which the other H-Y epitopes are derived. Another ubiquitously expressed Y chromosome gene, Uty, also mapping to this region, was found to express an H-YDb epitope. The H-YDb peptide is encoded in the 5' region of the Uty cDNA. It differs from that encoded by Utx, the X chromosome homologue of this gene, by three amino acids. The N residue at position 5 is the anchor residue and is required for binding to H-2Db, while M at position 6 and D at 7 are critical for T cell recognition. |
