| First Author | Nakano A | Year | 1993 |
| Journal | Immunol Lett | Volume | 39 |
| Issue | 1 | Pages | 77-82 |
| PubMed ID | 7511564 | Mgi Jnum | J:16641 |
| Mgi Id | MGI:64709 | Doi | 10.1016/0165-2478(93)90167-Z |
| Citation | Nakano A, et al. (1994) Induction of hypersensitivity to endotoxin in C3H/HeJ mice by immunization with L-form Salmonella typhimurium. Immunol Lett 39(1):77-82 |
| abstractText | When endotoxin low-responder C3H/HeJ mice were immunized with L-form Salmonella typhimurium, the mice were more susceptible to a lethal challenge with S. typhimurium 1 week after immunization (1-week mice) than were the unimmunized controls. One-week immune mice produced overwhelming amounts of tumor necrosis factor-alpha (TNF-alpha) in the blood after infection, while 4-week immune mice produced lesser amounts of this cytokine with a 75% survival rate at 60 days postinfection. Pretreatment with anti-TNF-alpha antibody prevented 1-week immune mice from succumbing to acute illness. Endotoxin-stimulated peritoneal macrophages from 1-week immune mice produced higher amounts of TNF-alpha in vitro than did those from 4-week immune mice and they expressed larger amounts of TNF-alpha mRNA on Northern blot. The capacity of macrophages to produce TNF-alpha in vitro was correlated with the degree of colonization by the L form in the cells. These results suggest that the colonization by L-form S. typhimurium in macrophages alters the susceptibility to S. typhimurium of C3H/HeJ mice and that TNF-alpha might play a major role in this alteration of host resistance. |
