| First Author | Guthmann M | Year | 2023 |
| Journal | Genes Dev | Volume | 37 |
| Issue | 7-8 | Pages | 336-350 |
| PubMed ID | 37072228 | Mgi Jnum | J:341518 |
| Mgi Id | MGI:7538788 | Doi | 10.1101/gad.350353.122 |
| Citation | Guthmann M, et al. (2023) A change in biophysical properties accompanies heterochromatin formation in mouse embryos. Genes Dev 37(7-8):336-350 |
| abstractText | The majority of our genome is composed of repeated DNA sequences that assemble into heterochromatin, a highly compacted structure that constrains their mutational potential. How heterochromatin forms during development and how its structure is maintained are not fully understood. Here, we show that mouse heterochromatin phase-separates after fertilization, during the earliest stages of mammalian embryogenesis. Using high-resolution quantitative imaging and molecular biology approaches, we show that pericentromeric heterochromatin displays properties consistent with a liquid-like state at the two-cell stage, which change at the four-cell stage, when chromocenters mature and heterochromatin becomes silent. Disrupting the condensates results in altered transcript levels of pericentromeric heterochromatin, suggesting a functional role for phase separation in heterochromatin function. Thus, our work shows that mouse heterochromatin forms membrane-less compartments with biophysical properties that change during development and provides new insights into the self-organization of chromatin domains during mammalian embryogenesis. |
