| First Author | Li Y | Year | 2023 |
| Journal | EMBO Rep | Volume | 24 |
| Issue | 2 | Pages | e55778 |
| PubMed ID | 36440627 | Mgi Jnum | J:347973 |
| Mgi Id | MGI:7638803 | Doi | 10.15252/embr.202255778 |
| Citation | Li Y, et al. (2023) M1AP interacts with the mammalian ZZS complex and promotes male meiotic recombination. EMBO Rep 24(2):e55778 |
| abstractText | Following meiotic recombination, each pair of homologous chromosomes acquires at least one crossover, which ensures accurate chromosome segregation and allows reciprocal exchange of genetic information. Recombination failure often leads to meiotic arrest, impairing fertility, but the molecular basis of recombination remains elusive. Here, we report a homozygous M1AP splicing mutation (c.1074 + 2T > C) in patients with severe oligozoospermia owing to meiotic metaphase I arrest. The mutation abolishes M1AP foci on the chromosome axes, resulting in decreased recombination intermediates and crossovers in male mouse models. M1AP interacts with the mammalian ZZS (an acronym for yeast proteins Zip2-Zip4-Spo16) complex components, SHOC1, TEX11, and SPO16. M1AP localizes to chromosomal axes in a SPO16-dependent manner and colocalizes with TEX11. Ablation of M1AP does not alter SHOC1 localization but reduces the recruitment of TEX11 to recombination intermediates. M1AP shows cytoplasmic localization in fetal oocytes and is dispensable for fertility and crossover formation in female mice. Our study provides the first evidence that M1AP acts as a copartner of the ZZS complex to promote crossover formation and meiotic progression in males. |
