| First Author | Choi SW | Year | 2024 |
| Journal | Biochem Biophys Res Commun | Volume | 699 |
| Pages | 149561 | PubMed ID | 38280307 |
| Mgi Jnum | J:345825 | Mgi Id | MGI:7595145 |
| Doi | 10.1016/j.bbrc.2024.149561 | Citation | Choi SW, et al. (2024) Calcium homeostasis modulator 2 (Calhm2) as slowly activating membrane current channel in mouse B cells. Biochem Biophys Res Commun 699:149561 |
| abstractText | In mouse B lymphocytes, an unidentified slow-activating voltage-dependent current resembling the characteristics of the Calhm family ion channel (I(Calhm-L)) was investigated. RT-PCR analysis revealed the presence of Calhm2 and 6 transcripts, with subsequent whole-cell patch-clamp studies indicating that the I(Calhm-L) is augmented by heat, alkaline pH, and low extracellular [Ca(2+)]. Overexpression of Calhm2, but not Calhm6, in N2A cells recapitulated I(Calhm-L). Moreover, Calhm2 knockdown in Bal-17 cells abolished I(Calhm-L). We firstly identify the voltage-dependent ion channel function of the Calhm2 in the mouse immune cells. ATP release assays in primary mouse B cells suggested a significant contribution of Calhm2 for purinergic signaling at physiological temperature. |
