| First Author | Liao Y | Year | 2024 |
| Journal | Stem Cell Reports | Volume | 19 |
| Issue | 3 | Pages | 399-413 |
| PubMed ID | 38428414 | Mgi Jnum | J:361245 |
| Mgi Id | MGI:7613523 | Doi | 10.1016/j.stemcr.2024.02.001 |
| Citation | Liao Y, et al. (2024) MSX1(+)PDGFRA(low) limb mesenchyme-like cells as an efficient stem cell source for human cartilage regeneration. Stem Cell Reports 19(3):399-413 |
| abstractText | Degenerative bone disorders have a significant impact on global health, and regeneration of articular cartilage remains a challenge. Existing cell therapies using mesenchymal stromal cells (MSCs) have shown limited efficacy, highlighting the necessity for alternative stem cell sources. Here, we have identified and characterized MSX1(+) mesenchymal progenitor cells in the developing limb bud with remarkable osteochondral-regenerative and microenvironment-adaptive capabilities. Single-cell sequencing further revealed the presence of two major cell compositions within the MSX1(+) cells, where a distinct PDGFRA(low) subset retained the strongest osteochondral competency and could efficiently regenerate articular cartilage in vivo. Furthermore, a strategy was developed to generate MSX1(+)PDGFRA(low) limb mesenchyme-like (LML) cells from human pluripotent stem cells that closely resembled their mouse counterparts, which were bipotential in vitro and could directly regenerate damaged cartilage in a mouse injury model. Together, our results indicated that MSX1(+)PDGFRA(low) LML cells might be a prominent stem cell source for human cartilage regeneration. |
