| First Author | Dueck A | Year | 2022 |
| Journal | bioRxiv | Mgi Jnum | J:346128 |
| Mgi Id | MGI:7614420 | Doi | 10.1101/2022.11.30.518576 |
| Citation | Dueck A, et al. (2022) The IncRNA landscape of cardiac resident macrophages and identification of Schlafenlnc as a regulator of macrophage migratory function. bioRxiv |
| abstractText | Cardiac resident macrophages (crMPs) were recently shown to exert pivotal functions in cardiac homeostasis and disease, but the underlying molecular mechanisms are largely unclear. Long non-coding RNAs (lncRNAs) are increasingly recognized as important regulatory molecules in a number of cell types, but neither the identity nor the molecular mechanisms of lncRNAs in crMPs are known. Here, we have employed deep RNA-seq and single cell RNA sequencing to resolve the crMP lncRNA landscape from healthy and diseased murine myocardium. CrMPs express previously unknown and highly cell type-specific lncRNAs, among which one lncRNA, termed Schlafenlnc, was particularly abundant and enriched in crMPs. We found Schlafenlnc to be necessary for migration-associated gene expression in macrophages in vitro and in vivo and essential for their adhesion and migration. Collectively, our data provide a basis to the systematic characterization of lncRNAs in crMPs and establish Schlafenlnc as a critical regulator of macrophage migratory functions. |
