| First Author | Schönberger K | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 2 | Pages | 101408 |
| PubMed ID | 35620073 | Mgi Jnum | J:349408 |
| Mgi Id | MGI:7646092 | Doi | 10.1016/j.xpro.2022.101408 |
| Citation | Schonberger K, et al. (2022) Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers. STAR Protoc 3(2):101408 |
| abstractText | Metabolism is important for the regulation of hematopoietic stem cells (HSCs) and drives cellular fate. Due to the scarcity of HSCs, it has been technically challenging to perform metabolome analyses gaining insight into HSC metabolic regulatory networks. Here, we present two targeted liquid chromatography-mass spectrometry approaches that enable the detection of metabolites after fluorescence-activated cell sorting when sample amounts are limited. One protocol covers signaling lipids and retinoids, while the second detects tricarboxylic acid cycle metabolites and amino acids. For complete details on the use and execution of this protocol, please refer to Schonberger et al. (2022). |
