| First Author | Wang X | Year | 2023 |
| Journal | Sci China Life Sci | Volume | 66 |
| Issue | 7 | Pages | 1459-1481 |
| PubMed ID | 37335463 | Mgi Jnum | J:350717 |
| Mgi Id | MGI:7664196 | Doi | 10.1007/s11427-023-2390-5 |
| Citation | Wang X, et al. (2023) The PIWI-specific insertion module helps load longer piRNAs for translational activation essential for male fertility. Sci China Life Sci 66(7):1459-1481 |
| abstractText | PIWI-clade proteins harness piRNAs of 24-33 nt in length. Of great puzzles are how PIWI-clade proteins incorporate piRNAs of different sizes and whether the size matters to PIWI/piRNA function. Here we report that a PIWI-Ins module unique in PIWI-clade proteins helps define the length of piRNAs. Deletion of PIWI-Ins in Miwi shifts MIWI to load with shorter piRNAs and causes spermiogenic failure in mice, demonstrating the functional importance of this regulatory module. Mechanistically, we show that longer piRNAs provide additional complementarity to target mRNAs, thereby enhancing the assembly of the MIWI/eIF3f/HuR super-complex for translational activation. Importantly, we identify a c.1108C>T (p.R370W) mutation of HIWI (human PIWIL1) in infertile men and demonstrate in Miwi knock-in mice that this genetic mutation impairs male fertility by altering the property of PIWI-Ins in selecting longer piRNAs. These findings reveal a critical role of PIWI-Ins-ensured longer piRNAs in fine-tuning MIWI/piRNA targeting capacity, proven essential for spermatid development and male fertility. |
