| First Author | Tanaka T | Year | 2024 |
| Journal | Sci Rep | Volume | 14 |
| Issue | 1 | Pages | 27192 |
| PubMed ID | 39516548 | Mgi Jnum | J:358454 |
| Mgi Id | MGI:7780967 | Doi | 10.1038/s41598-024-78683-x |
| Citation | Tanaka T, et al. (2024) Dermal macrophages control tactile perception under physiological conditions via NGF signaling. Sci Rep 14(1):27192 |
| abstractText | We demonstrated previously that sorting nexin 25 (SNX25) in nerve-associated macrophages plays critical roles in pain sensation by regulating tissue NGF content under both physiological and neuropathic conditions. In the present study, we apply the SNX25-NGF paradigm to tactile perception by showing that Snx25(+/-) mice or macrophage-specific Snx25 conditional knock-out (mcKO) mice had weaker responses to tactile stimuli in normal conditions. Snx25 mcKO mice responded poorly to transcutaneous electrical stimuli at a frequency of 5 Hz (C fiber responses), but normally to stimuli at a frequency of 250 Hz (Adelta fiber responses) or of 2000 Hz (Abeta fiber responses). CX3CR1-positive dermal macrophages were frequently found near calcitonin gene-related peptide (CGRP)- positive nerves and, less frequently, tyrosine hydroxylase (TH)-positive nerves. We confirmed that the tissue content of NGF was lower in Snx25 mcKO mice than in wild-type mice, and in turn, dermal NGF injection restored tactile sensitivity in Snx25(+/-) mice and Snx25 mcKO mice to normal levels. These results indicate that CGRP-positive C-nociceptors (possibly also TH-positive C-LTMRs) associated dermal macrophages control tactile perception by producing NGF and secreting it into the dermis. |
