| First Author | Huntley MA | Year | 2016 |
| Journal | BMC Genomics | Volume | 17 |
| Pages | 61 | PubMed ID | 26768488 |
| Mgi Jnum | J:356006 | Mgi Id | MGI:7762098 |
| Doi | 10.1186/s12864-015-2291-9 | Citation | Huntley MA, et al. (2016) Complex regulation of ADAR-mediated RNA-editing across tissues. BMC Genomics 17:61 |
| abstractText | BACKGROUND: RNA-editing is a tightly regulated, and essential cellular process for a properly functioning brain. Dysfunction of A-to-I RNA editing can have catastrophic effects, particularly in the central nervous system. Thus, understanding how the process of RNA-editing is regulated has important implications for human health. However, at present, very little is known about the regulation of editing across tissues, and individuals. RESULTS: Here we present an analysis of RNA-editing patterns from 9 different tissues harvested from a single mouse. For comparison, we also analyzed data for 5 of these tissues harvested from 15 additional animals. We find that tissue specificity of editing largely reflects differential expression of substrate transcripts across tissues. We identified a surprising enrichment of editing in intronic regions of brain transcripts, that could account for previously reported higher levels of editing in brain. There exists a small but remarkable amount of editing which is tissue-specific, despite comparable expression levels of the edit site across multiple tissues. Expression levels of editing enzymes and their isoforms can explain some, but not all of this variation. CONCLUSIONS: Together, these data suggest a complex regulation of the RNA-editing process beyond transcript expression levels. |
