| First Author | Wang J | Year | 2019 |
| Journal | Biol Proced Online | Volume | 21 |
| Pages | 20 | PubMed ID | 31636514 |
| Mgi Jnum | J:356085 | Mgi Id | MGI:7762177 |
| Doi | 10.1186/s12575-019-0108-y | Citation | Wang J, et al. (2019) Comprehensive Transcriptomic Analysis of Mouse Gonadal Development Involving Sexual Differentiation, Meiosis and Gametogenesis. Biol Proced Online 21:20 |
| abstractText | BACKGROUND: Mammalian gonadal development is crucial for fertility. Sexual differentiation, meiosis and gametogenesis are critical events in the process of gonadal development. Abnormalities in any of these events may cause infertility. However, owing to the complexity of these developmental events, the underlying molecular mechanisms are not fully understood and require further research. RESULTS: In this study, we employed RNA sequencing to examine transcriptome profiles of murine female and male gonads at crucial stages of these developmental events. By bioinformatics analysis, we identified a group of candidate genes that may participate in sexual differentiation, including Erbb3, Erbb4, and Prkg2. One hundred and two and 134 candidate genes that may be important for female and male gonadal development, respectively, were screened by analyzing the global gene expression patterns of developing female and male gonads. Weighted gene co-expression network analysis was performed on developing female gonads, and we identified a gene co-expression module related to meiosis. By alternative splicing analysis, we found that cassette-type exon and alternative start sites were the main forms of alternative splicing in developing gonads. A considerable portion of differentially expressed and alternatively spliced genes were involved in meiosis. CONCLUSION: Taken together, our findings have enriched the gonadal transcriptome database and provided novel candidate genes and avenues to research the molecular mechanisms of sexual differentiation, meiosis, and gametogenesis. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12575-019-0108-y. |
