| First Author | Ikeda H | Year | 2024 |
| Journal | STAR Protoc | Volume | 5 |
| Issue | 2 | Pages | 103050 |
| PubMed ID | 38703368 | Mgi Jnum | J:356249 |
| Mgi Id | MGI:7762341 | Doi | 10.1016/j.xpro.2024.103050 |
| Citation | Ikeda H, et al. (2024) Protocol for high-quality single-cell RNA-seq from tissue sections with DRaqL. STAR Protoc 5(2):103050 |
| abstractText | Single-cell RNA sequencing (scRNA-seq) combined with laser capture microdissection (LCM) offers a versatile framework for comprehensive transcriptomics from tissue sections. Here, we present a detailed protocol for DRaqL (direct RNA recovery and quenching for LCM) in combination with Smart-seq2 (DRaqL-Smart-seq2), which enables high-quality RNA sequencing for single cells obtained from alcohol-fixed murine ovarian sections. Additionally, we provide an optional procedure for scRNA-seq from formalin-fixed sections (DRaqL-Protease-Smart-seq2). We outline key steps for cell lysis, cDNA amplification, and sequencing library preparation. For complete details on the use and execution of this protocol, please refer to Ikeda et al.(1). |
