| First Author | Kim KX | Year | 2010 |
| Journal | Biochem Biophys Res Commun | Volume | 394 |
| Issue | 2 | Pages | 434-8 |
| PubMed ID | 20226170 | Mgi Jnum | J:356283 |
| Mgi Id | MGI:7762375 | Doi | 10.1016/j.bbrc.2010.03.048 |
| Citation | Kim KX, et al. (2010) Inward-rectifier chloride currents in Reissner's membrane epithelial cells. Biochem Biophys Res Commun 394(2):434-8 |
| abstractText | Sensory transduction in the cochlea depends on regulated ion secretion and absorption. Results of whole-organ experiments suggested that Reissner's membrane may play a role in the control of luminal Cl(-). We tested for the presence of Cl(-) transport pathways in isolated mouse Reissner's membrane using whole-cell patch clamp recording and gene transcript analyses using RT-PCR. The current-voltage (I-V) relationship in the presence of symmetrical NMDG-Cl was strongly inward-rectifying at negative voltages, with a small outward current at positive voltages. The inward-rectifying component of the I-V curve had several properties similar to those of the ClC-2 Cl(-) channel. It was stimulated by extracellular acidity and inhibited by extracellular Cd2+, Zn2+ and intracellular ClC-2 antibody. Channel transcripts expressed include ClC-2, Slc26a7 and ClC-Ka, but not Cftr, ClC-1, ClCa1, ClCa2, ClCa3, ClCa4, Slc26a9, ClC-Kb, Best1, Best2, Best3 or the beta-subunit of ClC-K, barttin. ClC-2 is the only molecularly-identified channel present that is a strong inward rectifier. This study is the first report of conductive Cl(-) transport in epithelial cells of Reissner's membrane and is consistent with an important role in endolymph anion homeostasis. |
