| First Author | Yang M | Year | 2023 |
| Journal | Ocul Surf | Volume | 30 |
| Pages | 263-275 | PubMed ID | 37769964 |
| Mgi Jnum | J:356795 | Mgi Id | MGI:7762887 |
| Doi | 10.1016/j.jtos.2023.09.012 | Citation | Yang M, et al. (2023) Genomic DNA activates the AIM2 inflammasome and STING pathways to induce inflammation in lacrimal gland myoepithelial cells. Ocul Surf 30:263-275 |
| abstractText | PURPOSE: Primary Sjogren's syndrome (pSS) is an autoimmune disease that mainly attacks the lacrimal glands causing severe aqueous-deficient dry eye. Clinical evidence indicates the DNA sensing mechanism in the pathogenesis of pSS. The purpose of the present study is to determine the pro-inflammatory effect of self-genomic DNA (gDNA) on myoepithelial cells (MECs), which along with acinar and ductal cells is a major cell type of the lacrimal gland. METHOD: MECs primary culture was acquired from female C57BL6J mice. Genomic DNA was extracted from the spleen of the same animal. The MECs were challenged with self-gDNA. The cytokine secretion was detected using supernatant by enzyme-linked immunosorbent assay (ELISA). The activation of inflammasomes was determined using FAM-FLICA. Cryosections of NOD.B10.H2(b) mouse model of pSS were obtained for immunofluorescence microscopy (IF), with Balb/C as control. RESULT: Treatment with gDNA activated AIM2 inflammasome assembly and function, leading to secretion of interleukin (IL)-1beta and IL-18 in MECs. The stimulation of IL-1beta secretion by gDNA appeared to be solely at the post-translational level, whereas IL-18 secretion was a combination of increased protein synthesis and post-translational modification. Genomic DNA also induced the activation of STimulators of INterferon Genes (STING), which correlated to the activation of STING in the lacrimal gland from the NOD.B10.H2(b) mouse. STING activation led to the secretion of IFN-beta via Nuclear Factor-kappaB (NF-kappaB). The IFN-beta further enhances the secretion of IL-1beta. The contractility of MECs was disabled by treatment with gDNA or poly AnT, independent of the level of intracellular [Ca(2+)]. CONCLUSION: Self-gDNA induces a proinflammatory response in lacrimal gland MECs by activating both the AIM2 inflammasome and STING and thus may contribute to the pathogenesis of pSS. |
