| First Author | Stelzl U | Year | 2005 |
| Journal | Cell | Volume | 122 |
| Issue | 6 | Pages | 957-68 |
| PubMed ID | 16169070 | Mgi Jnum | J:387750 |
| Mgi Id | MGI:8354403 | Doi | 10.1016/j.cell.2005.08.029 |
| Citation | Stelzl U, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell 122(6):957-68 |
| abstractText | Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and co-immunoprecipitation assays validated the overall quality of the Y2H interactions. Using topological and GO criteria, a scoring system was developed to define 911 high-confidence interactions among 401 proteins. Furthermore, the network was searched for interactions linking uncharacterized gene products and human disease proteins to regulatory cellular pathways. Two novel Axin-1 interactions were validated experimentally, characterizing ANP32A and CRMP1 as modulators of Wnt signaling. Systematic human protein interaction screens can lead to a more comprehensive understanding of protein function and cellular processes. |
