| First Author | Kim B | Year | 2025 |
| Journal | Biochem Biophys Res Commun | Volume | 742 |
| Pages | 151157 | PubMed ID | 39672006 |
| Mgi Jnum | J:360902 | Mgi Id | MGI:7851839 |
| Doi | 10.1016/j.bbrc.2024.151157 | Citation | Kim B, et al. (2025) alpha-Klotho regulates mouse embryonic neural stem cell proliferation and differentiation. Biochem Biophys Res Commun 742:151157 |
| abstractText | A longevity factor alpha-Klotho has been shown to be neuroprotective and enhance brain function. However, it has not been elucidated whether alpha-Klotho is involved in the embryonic brain development. To address this, we investigated whether alpha-Klotho regulates embryonic neural stem cell proliferation and differentiation. We found that alpha-Klotho is expressed in the mouse embryonic brain and neural stem cells isolated from the embryonic day 13.5 brains. When alpha-Klotho was knocked down in the embryonic neural stem cells, basal level of apoptosis increased while proliferation decreased. Overexpression of membrane-bound or secreted form of alpha-Klotho promoted proliferation of the mouse embryonic neural stem cells. Knockdown of alpha-Klotho suppressed the phosphorylation of Akt and ERK, suggesting that alpha-Klotho may promote neural stem cell survival and proliferation by activating signaling pathway involving Akt and ERK. In addition, knockdown of alpha-Klotho suppressed differentiation of embryonic neural stem cells into neurons or oligodendrocytes but not astrocytes. These results suggest that alpha-Klotho may have a role in neuronal development by promoting neural stem cell survival, proliferation and differentiation. |
