| First Author | Rasberry C | Year | 1993 |
| Journal | Mouse Genome | Volume | 91 |
| Pages | 856 | Mgi Jnum | J:23537 |
| Mgi Id | MGI:71344 | Citation | Rasberry C (1993) Three new reciprocal translocations: T(5;11)57H, T(3;12)58H, T(10;16)59H. Mouse Genome 91:856 |
| abstractText | Full text of Mouse Genome contribution: 5. Three new reciprocal translocations. We report here three new translocations produced by X-irradiation of spermatogonial stem cells. In each case the original carrier animal was a female which had been selected for genetic analysis on the basis of small size. Breeding tests showed that the size characteristic was not inherited but in each a semi-sterility suggested that a translocation might be present. This was confirmed through cytogenetic analysis of semi-sterile male descendants. T(5;11)57H Induced in stem cell spermatogonia of a (C3H/HeH x 101/H) Fl male given 2.0 mg/kg triethylenemelamine (TEM) and 1.0 ml of 0.1 M 3-aminobenzamide (24 h interval) followed 1 hr later by 6 Gy p.b X-irradiation. Metaphase I analysis of carrier males gave 57% RIV, 36% CHIV and 7% 20II. Mitotic breakpoints are in bands 5F/G or 5G2 and 11 A/B junction. T(3;12)58H Induced in stem cell spermatogonia of a (C3H/HeH x 101/H) Fl male given 2.0 mg/kg triethylenemelamine (TEM) followed 24 h later by 6 Gy p.b. X-irradiation. Metaphase I analysis of carrier males gave 43% RIV, 27% CHIV, 2% CHIII+I and 28% 20II. Mitotic breakpoints are in bands 3 E3/F and 12 C2. T(10;16)59H Induced as in T57H. Metaphase I analysis of carrier males gave 73% RIV and 27% CHIV. Mitotic breakpoints are in bands 10 CI and 16 B3. (Burtenshaw, Rasberry, Cattanach, Evans, Vizor and Woodward). (This work was supported in part by Euratom Contract F13P-CT920055). |
