| First Author | Brown LD | Year | 1978 |
| Journal | Fed Proc | Volume | 37 |
| Pages | 1584 (Abstr.) | Mgi Jnum | J:12935 |
| Mgi Id | MGI:61149 | Citation | Brown LD, et al. (1978) The expression of Lyb-2.1 on murine B lymphocytes. Fed Proc 37:1584 (Abstr.) |
| abstractText | Full text of Abstract. THE EXPRESSION OF LYB-2.1 ON MURINE B LYMPHOCYTES. Linda D. Brown*, F.W. Shen*, J.W. Uhr and E.S. Vitetta. University of Texas Southwestern Medical School, Dallas, Texas and Memorial Sloan-Kettering Cancer Center, New York, N.Y. The distribution of Lyb-2.1 antigen on functionally distinct populations of B lymphocytes was studied. Splenocytes from DBA/2J mice were radioiodinated and then treated with anti-Lyb-2.1 + C' + azide. Cells surviving the anti-Lyb-2.1 treatment were isolated, lysed, and the lysates precipitated with anti-Ig. Analysis of the immunoprecipitates on SDS-gels indicated that the surviving cells had a higher Mu/Delta ratio than the control cells. Since as B cells mature, the concentration of IgM on the cell surface decreases and/or the concentration of IgD increases, these results suggest that Lyb-2.1 is preferentially expressed on more mature B cells. In other experiments, surviving cells were cultured with TNP-Brucella (thymus-independent antigen) or TNP-SRBC (thymus-dependent antigen) and direct PFC assessed. The anti-TNP response to TNP-Brucella was the same as the control, while the response to TNP-SRBC was reduced by 65-85%. Previous experinents have shown that cells responding to TNP-Brucella bear predominantly IgM whereas cells responding to TNP-SRBC bear both IgM and IgD. Thus, these experiments also support the conclusion that Lyb-2.1 is expressed primarily on relatively mature B cells which bear both IgM and IgD. (Supported by NIH Grants AI-11851 and AI-12789.) |
