| First Author | Owerbach D | Year | 1978 |
| Journal | Hereditas | Volume | 89 |
| Pages | 146 (Abstr.) | Mgi Jnum | J:13365 |
| Mgi Id | MGI:61566 | Citation | Owerbach D, et al. (1978) Genetic determination of a parotid secretory protein in mouse. Hereditas 89:146 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of Papers. 17. Genetic determination of a parotid secretory protein in mouse. D. OWERBACH and J. P. HJORTH. Institute of Ecology and Genetics, University of Aarhus, DK-8000 Arhus C, Denmark. After an hour pulse, the mouse parotid glands incorporate 65-85% of the acid insoluble 3H-leucine label into a glycoprotein of approximately 20,000 molecular weight. Since this protein is only synthesized by the parotid gland and appears in saliva, it was named PSP (Parotid Secretory Protein). Pulse labelling of the mouse parotid glands, by injection of 35S-methionine into animals of inbred strains YBR/WiHa and C3H/A and separation of the proteins by SDS-polyacrylamide gel electrophoresis, reveals that PSP is labelled in the YBR strain but is not labelled in the C3H strain. Further analysis, by treatment with cyanogens bromide (CNBr) demonstrates that PSP(YBR) is degraded into 2 peptides of approximately 10,000 molecular weight while PSP(C3H) remains intact. In back- and intercrosses between YBR and C3H the expected classes for single gene codominant inheritance for this protein were obtained, which define the Psp locus. Examination of whole saliva by native gel electrophoresis provides an additional and more rapid method for phenotyping PSP. The CNBr sensitive type, PSP-A, is fast in native electrophoresis whereas the CNBr insensitive type, PSP-B, is slow. Examination of backcross and F2 progeny demonstrates that Psp is linked to the agouti locus on chromosome 2, at a distance of 6 +/- 4 cM, and that there is free recombination between Amy and Psp. In the YBR and C3H strains the protein ratio in whole saliva of amylase to PSP, analyzed by scans of native gels, reflects quantitative variation in salivary amylase production. The general application of using the salivary amylase/PSP ratio in screening for salivary amylase and PSP variants will be discussed. |
