| First Author | Abel K | Year | 1986 |
| Journal | Mouse News Lett | Volume | 74 |
| Pages | 108-109 | Mgi Jnum | J:20305 |
| Mgi Id | MGI:68405 | Citation | Abel K, et al. (1986) Mapping and expression analyses of murine angiotensinogen genes. Mouse News Lett 74:108-109 |
| abstractText | Full text of MNL contribution: Mapping and expression analyses of murine angiotensinogen genes. Using a homologous rat partial cDNA probe constructed by K. Lynch (University of Virginia School of Medicine, Charlottesville, Virginia), we have examined the structure and expression of the gene(s) encoding murine angiotensinogen. Restriction endonuclease analysis indicates that the genes within different inbred strains are highly conserved. However, the abundance of angiotensinogen-specific mRNA in liver varies markedly between inbred strains. Relative to males, DBA/2J females show an approximately 2-5-fold increase in liver mRNA abundance. Brain, testes and submaxillary gland show mRNA levels comparable to levels seen in liver in DBA/2J males, while the kidney levels are relatively reduced. Genomic DNAs of several inbred strains were screened for restriction site polymorphisms which could be used in chromosomal mapping studies. A SacI polymorphism was observed between DBA/2J (=D) and AKR/J (=A), and a limited survey of available Akx D recombinant inbred strains produced the following strain distribution pattern: RI# 1:D; 2:A; 3:D; 6:A; 7:A; 8:D; 9:D; 12:D; 13:A; 14:A; 15:D; 16:A; 18:D; 20:A; 21:A; 22:A; 23:A; 27:D. A search is continuing for structural differences in the angiotensinogen genes of various inbred strains to facilitate mapping. The ability of the rat probe to detect homologous sequences will permit the isolation of molecular clones from the mouse and permit a more detailed description of the structure and expression characteristics of this important component in the mechanics of blood pressure and electrolyte balance regulation. (R. Abel, K. Gross) |
