| First Author | Watanabe K | Year | 1992 |
| Journal | Mouse Genome | Volume | 90 |
| Issue | 3 | Pages | 444-45 |
| Mgi Jnum | J:2537 | Mgi Id | MGI:51059 |
| Citation | Watanabe K, et al. (1992) Mapping of mouse peptidylarginine deiminase gene (Pdi) to chr 4. Mouse Genome 90(3):444-45 |
| abstractText | Full text of Mouse Genome contribution: MAPPING OF MOUSE PEPTIDYLARGININE DEIMINASE GENE (Pdi ) TO CHR 4. Kazutada Watanabel, Naoki Maruyama2, and Rosemary W. Elliott3. 1Department of Experimental Biology, 2Department of Molecular Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173 Japan, and 3Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263 U.S.A. Peptidylarginine deiminase (PAD; EC3.5.3.15) is an enzyme which converts the guanido group of arginine residues in proteins to the ureido group of citrulline residues (1). We cloned a rat cDNA encoding PAD and defined its genomic structure (1, 2). The PAD spans more than 50 kb and contains 16 exons and 15 introns. We previously reported a restriction length fragment polymorphism (RFLP) in mouse peptidylarginine deiminase gene (Pdi ) (3). Based on the DNA variation between inbred strains after EcoRI digestion, we performed a segregation analysis of the alleles of Pdi using two sets of recombinant inbred (Rl) strains, BXD and AKXL (4). As shown in Table 1, the locus lies between Akp-2 and Gpd-1 (5). There is one recombinant with Akp-2 among 41 RI strains typed, indicating a map distance of 0.63 cM with 95% confidence limits at 0.02 and 3.98 cM (6). The 5/41 recombinants with Gpd-1 indicate a map distance of 3.73 cM with 95% confidence limits at 1.09 and 10.79 cM (6). These results suggest that Pdi lies on the distal end of Chr 4, between Akp-2 and Gpd-1 (Figure 1). Since Pdi is located in a conserved segment between mouse and human, one can expect that the human equivalent is located on human chromosome 1 (7). References 1. Watanabe, K. and Senshu, T. (1989) J. Biol. Chem. 264:15255. 2. Watanabe, K., Nomoto, M., Nagata, S., Itoh, Y., Hikichi, K., Maruyama, N., Mita, T., and Senshu, T. (1992) Gene 114:261. 3. Maruyama, N., Handa, S. and Watanabe, K. (1991) Mouse Genome 89:278. 4. Wilcox, F.H. and Taylor, B.A. (1981) J. Hered. 72:387. 5. Taylor, B.A., Bedigian, H.G., and Meier, H. (1977) J. Virol. 23:106. 6. Silver, J. (1985) J. Hered. 78:436. 7. Lyon, M.F. and Kirby, M.C. (1992) Mouse Genome 90:22. Table 1. Strain distribution pattern of alleles of Pdi and linked loci in the BXD and AKXL Rl strains. Strain Locus: Akp-2; 1: D; 2: B; 5: B; 6: B; 8: D; 9: B; 11: B; 12: D; 13: D; 14: D; 15: D; 16: D; 18: D; 19: B; 20: B; 21: D; 22: B; 23: D; 24: B; 25: B; 27: B; 28: D; 29: B; 30: D; 31: B; 32: D. Strain Locus: Gpd-1; 1: D; 2: B; 5: D; 6: B; 8: D; 9: B; 11: B; 12: D; 13: D; 14: D; 15: B; 16: D; 18: D; 19: B; 20: B; 21: D; 22: B; 23: B; 24: B; 25: B; 27: B; 28: D; 29: B; 30: D; 31: B; 32: D. Strain Locus: Akp-2; 5: L; 6: A; 7: A; 8: A; 9: L; 12: A; 13: L; 14: A; 16: L; 17: A; 19: A; 21: L; 24: L; 25: L; 28: L; 29: A; 37: L; 38: A. Strain Locus: Pdi; 5: L; 6: A; 7: A; 8: L; 9: A; 12: A; 13: L; 14: A; 16: L; 17: A; 19: A; 21: L; 24: L; 25: L; 28: L; 29: A; 37: L; 38: A. Strain Locus: Gpd-1; 5: L; 6: A; 7: A; 8: L; 9: A; 12: A; 13: L; 14: L; 16: L; 17: A; 19: A; 21: A; 24: L; 25: L; 28: L; 29: L; 37: A; 38: A. A, B, D, and L are genetic symbols for alleles inherited from AKR/J, C57BL/6, DBA/2, and C57L/J, respectively. U represents undetermined. A crossover between two loci is indicated by an X. Figure 1. (Legend). Location of Pdi locus on murine chromosome 4. |
