| First Author | Paige AJW | Year | 1997 |
| Journal | Genet Res | Volume | 70 |
| Pages | 85 (Abstr.) | Mgi Jnum | J:44122 |
| Mgi Id | MGI:1099373 | Citation | Paige AJW, et al. (1997) Genetic and physical mapping in the vicinity of the wi locus on mouse Chromosome 4. Genet Res 70:85 (Abstr.) |
| abstractText | Full text of Abstract: Genetic and physical mapping in the vicinity of the wi locus on mouse chromosome 4. A.J.W. PAIGE1, A.VARELA1, S.D.M. BROWN1, B.W. KIERNAN2, J. FLEMING2, M. ROGERS2, D. HUGHES2 and K.P. STEEL2. 1MRC Mouse Genome Centre, Harwell, Oxfordshire, OX11 ORD; 2MRC Institute of Hearing Research, Nottingham NG7 2RD, UK. Whirler (wi) is a spontaneously arising mutation in mouse which results in deafness and vestibular dysfunction. Electrophysiological studies showed impaired cochlear potentials which degenerate over time, and electron microscopy analysis of the inner ear revealed abnormal development of the inner hair cells. High resolution genetic maps in the vicinity of the wi locus have been constructed using large interspecific and intraspecific backcrosses segregating the wi mutation. The backcross progeny were genotyped with all the available markers on central mouse chromosome 4 and wi was found to be flanked by Ambp/D4Mit87 and Tnc, mapping 0.13 cM proximal and 0.39 cM distal, respectively of wi. The gene Orm1 has been shown to be non-recombinant with wi in both crosses. In addition, the European Collaborative Interspecific Backcross (EUCIB) was used to generate a high-resolution genetic map of microsatellites across the wi region. We have successfully constructed a YAC clone contig across the wi region using a combination of TAIL PCR and vectorette PCR to recover YAC end clones. Bl IRS PCR has been used to recover new internal STSs to further define the wi critical region. In addition, a P1 clone contig is being constructed around Orm1 and these clones are being used for a combination of exon trapping cDNA selection and sample sequencing to identify possible candidate genes for the wi mutation. |
