| First Author | Zabel BU | Year | 1984 |
| Journal | Cytogenet Cell Genet | Volume | 37 (1-4) |
| Pages | 614-615 (Abstr.) (399-616) | Mgi Jnum | J:12530 |
| Mgi Id | MGI:60775 | Citation | Zabel BU, et al. (1984) The nerve growth factor (Ngf) gene is on mouse chromosome 3 (Abstracts of meeting presentations: Human gene mapping 7, Los Angeles Conference (1983) Seventh International Workshop on Human Gene Mapping). Cytogenet Cell Genet 37 (1-4):614-615 (Abstr.) (399-616) |
| abstractText | Full text of Abstract: Abstracts of meeting presentations. PAGE 614. The nerve growth factor (Ngf) gene is on mouse chromosome 3. B.U. Zabel,1 A.Y. Sakaguchi,1 P.A. Lalley,2 J. Scott,3 and S.L. Naylor1. 1Roswell Park Memorial Institute, Buffalo, NY, 2Oak Ridge National Laboratory, Oak Ridge, TN, and 3Molecular Medicine Group, MRC, Clinical Research Center, Harrow, Middlesex, HA1 307. Nerve growth factor (NGF, also referred to as the betaNGF) is a protein essential for neuron development. High levels of NGF activity are expressed in the adult male mouse submaxillary gland. A 1.1 kb cDNA from this tissue encoding the mouse NGF precursors (prepro NGF) was used to chromosomally assign the mouse Ngf gene, by the methodologies of Southern filter hybridization and mouse-Chinese hamster somatic cell hybrids. The cDNA probe hybridized to 15.5 kb and 7 kb fragments of BALB/c mouse DNA cleaved with HindIII, whereas HindIII cleaved Chinese hamster DNA yielded 14.5 kb and 5.5 kb hybridizing fragments. DNAs from 15 mouse-Chinese hamster cell hybrids were isolated and hybridized to the NGF probe. The mouse chromosome content of each hybrid line was determined by analysis of cell homogenates for mouse enzyme markers and by karyotyping. Mouse Ngf segregated concordantly only with mouse chromosome 3. This segregation pattern was identical to that of mouse amylase when these cell hybrid DNAs were annealed with a rat amylase probe. Mouse amylase (Amyl, Amy2) is known to be on mouse chromosome 3 (E.M. Eicher and P.W. Lane, J. Hered. 71: 315-18, 1980). The data suggest that mouse Amy1, Amy2, and Ngf reside in a conserved linkage group, as the human AMYl, AMY2, and Ngf are all located on the short arm of chromosome 1. |
