| First Author | Naylor SL | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 669 (Abstr.) | Mgi Jnum | J:12366 |
| Mgi Id | MGI:60614 | Citation | Naylor SL, et al. (1987) The genes for mouse plasminogen activator and urokinase map to Chromosomes 8 and 14, respectively. Cytogenet Cell Genet 46:669 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. The genes for mouse plasminogen activator and urokinase map to chromosomes 8 and 14, respectively. Naytor SL, Marshall A, Killary AM, Lalley PA, Belin D, Rickles R, Strickland S, Rajput B. University of Texas Health Science Center, San Antonio, TX. Institute for Medical Research, Bennington, VT. Institut d'Histologie et d'Embryologie, Center Medical Universitaire, Geneva, Switzerland. State University of New York at Stonybrook, Stonybrook, NY. Friedrich Miescher-Institut, Basel, Switzerland. Plasminogen activators convert plasminogen, an inactive proenzyme in plasma, to plasmin, a protease involved in fibrinolysis and other cell processes. Two immunologically distinct plasminogen activators have been identified in mammals: tissue type plasminogen activator and urokinase. Analysis of the amino acid and nucleotide sequences of these genes indicate that they are homologous, but separate genes. In human the genes map to chromosome 8 for tissue plasminogen activator and chromosome 10 for urokinase. Comparative mapping in the mouse was done to determine if the genes lie in conserved synteny groups. A panel of mouse-Chinese hamster hybrids was used to map these genes initially. The urokinase probe hybridized to a 4.6 kb BamHI fragment in mouse DNA and a 10 kb fragment of Chinese hamster DNA. The 4.6 kb fragment segregated in 16 cell hybrids with chromosome 14. Additionally 4 mouse-rat hybrids were analyzed with a EcoRI digest and showed concordant segregation with chromosome 14. The tissue plasminogen activator probe hybridized with a 8.5 kb BamHI fragment of mouse genomic DNA. A BamHI digest of mouse-Chinese hamster hybrids did not unequivocally assign tissue plasminogen activator to a chromosome, but mouse-rat microcell hybrids were used to assign the tissue plasminogen activator to chromosome 8. Microcell F(8)D, which contains only chromosome 8, hybridized with the tissue plasminogen activator probe, whereas FB(8)D, which selectively lost chromosome 8, did not. Tissue type plasminogen activator appears to be in a conserved synteny group with glutathione reductase, while urokinase and adenosine kinase may lie in a synteny group. Other markers for both of these chromosomes will define the extent of conservation. |
