| First Author | Leiter EH | Year | 1993 |
| Journal | Mouse Genome | Volume | 91 |
| Issue | 3 | Pages | 567-568 |
| Mgi Jnum | J:14809 | Mgi Id | MGI:62970 |
| Citation | Leiter EH, et al. (1993) The rhodanese (Tst) gene maps to mouse chromosome 15 (Erratum: 1995; 93(3):863). Mouse Genome 91(3):567-568 |
| abstractText | Full text of Mouse Genome contribution: THE RHODANESE (Tst) GENE MAPS TO MOUSE CHROMOSOME 15. Edward H. Leiterl, Harold D. Chapmanl, and Thomas P. Dooley2, The Jackson Laboratory, Bar Harbor, ME, 04609, and Dept. of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78228 2, USA Rhodanese (EC 2.8.1.1) is a mitochondrial thiosulfate sulfurtransferase considered to be important in iron-sulfur cluster formation and detoxification of cyanide. The enzyme is encoded by a nuclear gene and functions to prevent cyanide inhibition of mitochondrial electron transport by covalently transferring sulfur atoms to cyanide, producing thiocyanate. The structural gene for bovine and rat rhodanese has been cloned [1, 2]. Comparison of the cDNA open reading frames from these two mammalian genera indicates 91% identity at the amino acid sequence level [2]. We have used the rat rhodanese cDNA to map the mouse homolog (provisionally Tst, for thiosulfate sulfurtransferase, mitochondrial) to Chromosome 15. Materials and Methods The Tst allele in C57BL/6J (B6) genomic DNA was distinguished by Southern blot from that of inbred Mus spretus, SPRET/Ei (SPRET) by hybridization with pRhoD, a 1 kb probe encompassing the 885 bp open reading frame of rat liver rhodanese cDNA [2]. Chromosomal mapping was performed by analysis of a genetically-characterized panel of 94 progeny DNAs from a (B6 x SPRET)F1 x SPRET first generation backcross. The B6 allele at the Tst locus was distinguished from the SPRET allele by the presence in B6 but not in SPRET of a 4.4 kb restriction fragment following Bam HI digestion. The DNA typing panel was obtained from the Genetic Mapping Resource of The Jackson Laboratory. Southern blots were prepared using standard procedures; filters were washed 3X in 0.1XSSC plus 0.1% SDS at 50 degrees C for 30 min/wash. The typing results were entered into The Jackson Laboratory Gene Mapping Resource database for identification of linkage association with previously mapped polymorphic loci in this DNA panel. In addition to loci typed by PCR using MIT microsatellite sequences [3], as well as endogenous proviruses [4], this database contains a number of loci identified on the basis of motif sequence-tagged PCR products distinguishing the B6 from the SPRET genome [5]. A description of this mapping panel is currently being prepared for publication (L. Rowe and E.H. Birkenmeier, The Jackson Laboratory, personal communication). Results and Discussion The mouse Tst gene maps to the central portion of Chr 15 between D15Mit3 (currently positioned at 18 cM) and D15Mit16 (currently positioned at 45 cM) as indicated by linkage to two Chr. 15 marker loci, Pmv-17 and D15Bir17 (Table I). Pmv-17 is an endogenous polytropic murine leukemia proviral locus positioned 26 cM from the centromere. There were 1/50 recombinants between this marker locus and Tst. D15Bir17 is a motif sequence-tagged PCR product previously mapped in this DNA panel to Chr. 15 (L. Rowe and E.H. Birkenmeier, The Jackson Laboratory, unpublished results). When the interval between Tst and D15Birl7 was analyzed, only 1 recombinant was found in 92 interspecific backcross progeny. Since 1/50 recombinants were found between Pmv-17 and D15Birl7, the presumed gene order is D15Mit3 -Pmv-17-Tst-D15Birl7-D15Mitl6. Table I. Mapping of Tst (thiosulfate sulfurtransferase, mitochondrial = rhodanese) to Chr 7 in a (B6 x SPRET) x SPRET BC1 typing panel. Interval: D15Mit3-Tst; recombinants/total: 7/93; recombinant frequency (x 100) +/- sem: 7.5 +/- 2.7; 95% confidence limits (lower-upper): 2-13 cM. Interval: Pmv-17-Tst; recombinants/total: 1/50; recombinant frequency (x 100) +/- sem: 2.0 +/- 2.0; 95% confidence limits (lower-upper): 1-16 cM. Interval: Tst-D15Bir17-; recombinants/total: 1/92; recombinant frequency (x 100) +/- sem: 1.1 +/- 1.1; 95% confidence limits (lower-upper): 0-6 cM. Interval: D15Bir17-D15Mit16; recombinants/total: 12/91; recombinant frequency (x 100) +/- sem: 13.2 +/- 3.6; 95% confidence limits (lower-upper): 6-20 cM. Acknowledgements. This work was supported by National Institutes of Heath Grant DK36175 and a grant from the Diabetes Research and Education Foundation (EHL) and a grant from the Southwest Foundation Forum (TPD). Dr. Edward Birkenmeier and Ms. Lucy Rowe are gratefully acknowledged for providing The Jackson Laboratory Gene Mapping Resource DNA panels, and for providing unpublished data from this panel. Dr. Wayne Frankel and Verity Letts of The Jackson Laboratory are acknowledged for providing the Pmv-17 typing. Ms. Dean Turner of The Jackson Laboratory provided the MIT microsatellite typing for this DNA panel. References. 1. D. Miller et al. (1991) J. Biol. Chem. 266: 4686-4691 2. K. Weiland & T. Dooley (1991) Biochem. J. 275: 227-231 3. W. Dietrich et al. (1992) Genetics 131: 423-447 4. W. Frankel et al. (1990) Genetics 124: 221-236. 5. E. Birkenmeier et al. (1992) Mamm. Genome 3: 537-545 ERRATUM MOUSE GENOME, VOL. 91 (3), SEPTEMBER, 1993; pp 567-568. The Rhodanese (Tst) Gene Maps to Mouse Chromosome 15. Edward H. Leiter, Harold D. Chapman, and Thomas P. Dooley. In mapping the Tst gene to Chr. 15, the (B6 x SPRET) x SPRET backcross panel of DNAs from The Jackson Laboratory Backcross DNA Panel Map Service was used. In our submission, recombination between Tst and a distal marker D15Bir17 was reported. When these data were generated, the locus then designated D15Birl7 was shown in the database as proximal to D15Mit16, and this nomenclature was employed in our presentation of recombination data. However, after our data were published in Mouse Genome, the formal publication describing the database was published by Rowe et al. [Maps from two interspecific backcross DNA panels as a community mapping resource. Mammal. Genome; 5 :253-274 (1994)]. In the publication by Rowe et al., the locus identified as D15Bir17 in our communication had been renamed D15Bir16. In view of this change, our Table 1 should be modified as shown below. Table I. Mapping of Tst (thiosulfate sulfurtransferase, mitochondria1 = rhodanese) to Chr 15 in a (B6 x SPRET) x SPRET BC1 typing panel. Interval: D15Mit3-Tst; recombinants/total: 7/93; recombinant frequency (x 100) +/- sem: 7.5 +/- 2.7; 95% confidence limits (lower-upper): 2-13 cM. Interval: Pmvl7-Tst; recombinants/total: 1/50; recombinant frequency (x 100) +/- sem: 2.0 +/- 2.0; 95% confidence limits (lower-upper): 1-16 cM. Interval: Tst-D15Birl6-; recombinants/total: 1/92; recombinant frequency (x 100) +/- sem: 1.1 +/- l.l; 95% confidence limits (lower-upper): 0-6 cM. Interval: D15Birl6-D15Mitl6; recombinants/total: 12/91; recombinant frequency (x 100) +/- sem: 13.2 +/- 3.6; 95% confidence limits (lower-upper): 6-20 cM. |
